Peptide Dose Calculator &
Complete Reconstitution Guide
Calculate your dose in seconds. Learn proper storage, reconstitution, and injection techniques. Everything you need to prepare peptides accurately—in one place.
🧮 Peptide Dose Calculator
Select a compound or enter values manually.
Peptide in vial (2mg, 5mg, 10mg)
Water added (typically 1-3ml)
Dose per injection
DRAW THIS MUCH
10
units on insulin syringe
Concentration
2.50
mg/ml
Doses Per Vial
20
at 250mcg/dose
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❄️Peptide Storage Guide
Proper storage is critical for peptide stability. Temperature, light, and humidity can all degrade your peptides, reducing effectiveness or rendering them useless.
📦 Lyophilized (Powder Form)
- •Refrigerator (2-8°C / 36-46°F): 2-3 years shelf life
- •Freezer (-20°C / -4°F): 3-5+ years shelf life
- •Room temperature: 1-3 months (not recommended)
💧 Reconstituted (Mixed)
- •Refrigerator only: 2-4 weeks typical
- •Never freeze reconstituted peptides
- •Keep away from light — wrap in foil if needed
⚠️ Signs of Degradation: Cloudy solution, particles floating, unusual color, or clumping. If you see any of these, discard the vial—degraded peptides may be ineffective or harmful.
📦Supplies Needed
Before you start, make sure you have all the supplies ready. Using the right equipment ensures accurate dosing and sterility.
Bacteriostatic Water
EssentialSterile water with 0.9% benzyl alcohol preservative. Allows multi-use over weeks.
Insulin Syringes (U-100)
Essential29-31 gauge, 0.5ml or 1ml. The most common choice for peptide injection.
Alcohol Swabs
EssentialFor cleaning vial tops and injection sites. 70% isopropyl alcohol.
Sharps Container
EssentialProper disposal of used needles. Never reuse or discard in regular trash.
Mixing Syringe (optional)
Larger syringe (3ml) for adding bac water. Can use insulin syringe instead.
Peptide Storage Box
Light-blocking container for refrigerator storage.
💉Understanding Insulin Syringes
Insulin syringes are measured in "units" designed for U-100 insulin (100 units per ml). For peptides, we use this same scale:
U-100 Syringe Scale
10
units = 0.1 ml
25
units = 0.25 ml
50
units = 0.5 ml
100
units = 1 ml
Choosing the Right Syringe
0.3ml (30 unit)
Best for very small doses. Easier to read small increments.
0.5ml (50 unit) ⭐
Most versatile. Recommended for most peptide protocols.
1ml (100 unit)
For larger doses or mixing. Markings harder to read for small doses.
💡 Needle Gauge: Higher gauge = thinner needle. 29-31 gauge is standard for subcutaneous peptide injection. 31G is nearly painless but may take longer to draw.
🧪Reconstitution Step-by-Step
Reconstitution is mixing your lyophilized (powder) peptide with bacteriostatic water to create an injectable solution. Follow these steps carefully.
Prepare Your Workspace
Clean surface. Wash hands. Have all supplies ready. Remove peptide vial from refrigerator 5-10 minutes before to reach room temperature.
Clean Vial Tops
Wipe the rubber stoppers of both the peptide vial and bacteriostatic water vial with alcohol swabs. Let dry for 10-15 seconds.
Draw Bacteriostatic Water
Draw your desired amount of bac water (1-3ml typical). Pull back plunger, insert needle into water vial, invert, draw water, remove air bubbles.
Add Water to Peptide (SLOWLY)
Insert needle into peptide vial at an angle. Let water drip down the side of the vial—do NOT spray directly onto the powder. This can damage the peptide.
Gently Swirl
Do NOT shake. Gently roll or swirl the vial to dissolve the powder. Most peptides dissolve within 30-60 seconds. Some may take longer.
Inspect Solution
Solution should be clear with no particles. If cloudy or has visible debris, the peptide may be degraded. Write the reconstitution date on the vial.
⚠️ Never Shake: Vigorous shaking can denature the peptide, breaking its molecular structure and rendering it ineffective.
💪Subcutaneous (SubQ) Injection
Subcutaneous injection delivers the peptide into the fat layer just below the skin. This is the most common method for peptides like BPC-157, TB-500, and most growth hormone secretagogues.
Common Injection Sites
- Abdomen:2 inches from navel. Most common site. Rotate sides.
- Thigh:Front/outer thigh. Easy to self-administer.
- Upper Arm:Back of upper arm. May need assistance.
- Love Handles:Side fat above hip. Good for rotation.
Injection Technique
- 1. Clean site with alcohol swab, let dry
- 2. Pinch skin to create a fold
- 3. Insert needle at 45-90° angle
- 4. Release pinch, inject slowly
- 5. Wait 5 seconds, withdraw needle
- 6. Apply gentle pressure (no rubbing)
💡 Tip: Rotate injection sites to prevent lipohypertrophy (hardened fat deposits). Never inject into the same spot repeatedly.
🦵Intramuscular (IM) Injection
Intramuscular injection delivers directly into muscle tissue. Used for some peptides (like certain HGH protocols) and most testosterone/hormone injections. Requires a longer needle than SubQ.
⚠️ Note: Most peptides are administered subcutaneously, NOT intramuscularly. Check your specific protocol. IM is primarily for oil-based compounds and certain hormones.
Common IM Sites
- Deltoid:Upper arm muscle. Small volume only (≤1ml).
- Vastus Lateralis:Outer thigh. Easy to self-administer.
- Ventrogluteal:Hip area. Safest for larger volumes.
- Gluteus Maximus:Buttock. Requires assistance.
IM Requirements
- • Needle length: 1-1.5 inch (25mm-38mm)
- • Gauge: 22-25G (thicker than SubQ)
- • Angle: 90° straight into muscle
- • Aspiration: Optional (pull back to check for blood)
❓Frequently Asked Questions
Can I use sterile water instead of bacteriostatic water?
Sterile water can be used but must be used within 24 hours and cannot be stored. Bacteriostatic water contains a preservative that allows multi-use over 2-4 weeks.
How do I know if my peptide has gone bad?
Signs include: cloudy or discolored solution, visible particles or clumping, unusual smell, or significantly reduced effects. When in doubt, discard.
Can I travel with reconstituted peptides?
Reconstituted peptides must stay refrigerated. For travel, use a cooler with ice packs. Extended time without refrigeration (>4-6 hours) may degrade the peptide.
Why are my injection sites getting lumps?
This is likely lipohypertrophy from injecting in the same spot repeatedly. Rotate injection sites. Existing lumps may take months to resolve.
Is it normal for the injection to sting?
Mild stinging is normal, especially with certain peptides or if the bac water is cold. Severe pain, swelling, or redness may indicate infection—consult a healthcare provider.
How do I dispose of used needles?
Never put needles in regular trash. Use a sharps container. When full, many pharmacies accept them for disposal, or check local regulations for drop-off locations.
Track Your Protocols with DosingIQ
The calculator is just the beginning. Upload your bloodwork, log your doses, and let AI find patterns in YOUR data—free to start.
Disclaimer: This guide is for educational purposes only. This is not medical advice. Consult a licensed healthcare provider before using any peptide, hormone, or supplement protocol. DosingIQ does not sell peptides or provide medical recommendations.